|
KMID : 0380020040190010042
|
|
Korean Journal of Biotechnology and Bioengineering
2004 Volume.19 No. 1 p.42 ~ p.49
|
|
|
Molecular Characterization of Epoxide Hydrolase from Aspergillus niger LK using Phylogenetic Analysis
|
|
Kim Hee-Sook
Lee Eun-Yeol
Lee Soo-Jung
Lee Ji-Won
|
|
|
Abstract
|
|
|
|
A gene coding for epoxide hydrolase (EH) of Aspergillus niger LK, a fungus possessing the enantioselective hydrolysis activity for racemic epoxides, was characterized by phylogenetic analysis. The deduced protein of A. niger LK epoxide hydrolase shares significant sequence similarity with several bacterial EHs and mammalian microsomal EHs (mEH) and belongs to the a/ hydrolase fold family. EH from A. niger LK had 90.6% identity with 3D crystal structure of lqo7 in Protein Data Bank. Sequence comparison with other source EHs suggested that Aspl92/, Asp374/ and His374/ constituted the catalytic triad. Based on the multiple sequence comparison of the functional and structural domain sequence, the phylogenetic tree between relevant epoxide hydrolases from various species were reconstructed by using Neighbor-Joining method. Genetic distances were so far as 1.841-2.682 but characteristic oxyanion hole and catalytic triad were highly conserved, which means they have diverged from a common ancestor.
|
|
|
KEYWORD
|
|
|
Aspergillus niger, enantioselective hydrolysis, epoxide hydrolase, phylogenetic analysis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|